AG2R Prevoyance, France
- Eighth Tour appearance, six stage victories
- Bike frame: b'Twin Racing (Décathlon)
- Riders to watch: Christophe Moreau, Cyril Dessel
Agritubel, France
- Second Tour appearance, one stage victory
- Bike frame: MBK
- Riders to watch: Juan Miguel Mercado, Nicolas Jalabert
Astana, Switzerland
- First Tour appearance
- Bike frame: BMC
- Riders to watch: Alexandre Vinokourov, Andreas Kloden, Paolo Savoldelli
Barloworld, South Africa
- First Tour appearance
- Bike frame: Cannondale
- Riders to watch: Robert Hunter, Felix Cardenas
Bouygues Telecom, France
- Third Tour appearance, one stage victory
- Bike frame: Time
- Riders to watch: Thomas Voeckler, Pierrick Fedrigo
Caisse D'Epargne, Spain
- 18th Tour appearance, 16 stage victories, five individual overall victories
- Bike frame: Pinarello
- Riders to watch: Alejandro Valverde, Oscar Pereiro
Cofidis, France
- 11th Tour appearance, eight stage victories
- Bike frame: Time
- Riders to watch: Bradley Wiggins, Sylvain Chavanel
Credit Agricole, Spain
- Ninth Tour appearance, six stage victories
- Bike frame: Look
- Riders to watch: Thor Hushovd, Sébastien Hinault
CSC, Denmark
- Seventh Tour appearance, nine stage victories
- Bike frame: Cervélo
- Riders to watch: Carlos Sastre, Fabian Cancellera, Jens Voigt, Stuart O'Grady
Discovery Channel, U.S.A.
- 11th Tour appearance, 26 stage victories, seven individual overall victories
- Bike frame: Trek
- Riders to watch: Levi Leipheimer, George Hincapie, Alberto Contador, Yaroslav Popovych
Euskaltel-Euskadi, Spain
- Seventh Tour appearance, two stage victories
- Bike frame: Orbea
- Rider to watch: Haimar Zubeldia
Francaise des Jeux, France
- 11th Tour appearance, four stage victories
- Bike frame: Lapierre
- Riders to watch: Sandy Caser, Thomas Lovkvist
Gerolsteiner, Germany
- Fifth Tour appearance, one stage victories
- Bike frame: Specialized
- Rider to watch: Markus Fothen
Lampre-Fondital, Italy
- Third Tour appearance
- Bike frame: Wilier
- Riders to watch: Paolo Bossoni, Alessandro Ballan
Liquigas, Italy
- Third Tour appearance
- Bike frame: Cannondale
- Riders to watch: Filippo Pozzato, Manual Quinziato
Milram, Italy
- Second Tour appearance
- Bike frame: Colnago
- Rider to watch: Erik Zabel
Predictor-Lotto, Belgium
- Third Tour appearance, six stage victories
- Bike frame: Ridley
- Riders to watch: Cadel Evans, Robbie McEwen, Christopher Horner, Fred Rodriguez
Quick Step-Innergetic, Belgium
- Fifth Tour appearance, nine stage victories
- Bike frame: Specialized
- Rider to watch: Tom Boonen
Rabobank, Netherlands
- 12th Tour appearance, 18 stage victories
- Bike frame: Colnago
- Riders to watch: Michael Rasmussen, Oscar Freire, Denis Menchov
Saunier Duval-Prodir, Spain
- Third Tour appearance
- Bike frame: Scott
- Riders to watch: David Millar, Ibán Mayo
T-Mobile, Germany
- 16th Tour appearance, 30 stage victories, two individual overall victories
- Bike frame: Giant
- Riders to watch: Michael Rogers, Axel Merckx